Scientists have discovered the first known hybrid bird species to be found in the Amazon rainforest - a golden-crowned manakin with yellow feathers.
Through a series of genetic and other tests researchers found that the golden-crowned manakin - first discovered in Brazil in 1957 but not seen again until 2002 - is in fact a hybrid species.
"While hybrid plant species are very common, hybrid species among vertebrates are exceedingly rare," said Jason Weir, from University of Toronto in Canada.
A hybrid species forms when two parental species mate to produce a hybrid population, which then stops being able to freely interbreed with the parental species.
In this case the two parents are the snow-capped manakin, named for its bright snowy-white crown feathers, and the opal-crowned manakin, named for its brilliant iridescent crown feathers.
Researchers gathered genetic and feather samples over two separate field trips to Brazil.
They were then able to sequence a large portion of the golden-crowned manakins genome including 16,000 different genetic markers, finding that about 20 per cent of its genome came from the snowy-crowned, and about 80 per cent came from the opal-crowned.
The researchers also used something called coalescent modelling to figure out at what point the golden-crowned split off from its parental species.
They determined it was around 180,000 years ago when the two parental species originally mated, and that both parental species diverged from a common ancestor about 300,000 years ago, making all three very recent birds by Amazon rainforest standards.
"Most Amazon bird species diverged from their most recent relative around 1.5 to 4 million years ago, so these are all young birds by comparison" said Weir.
The male golden-crowned has unique yellow crown feathers that are much duller than its parental species. To learn more about this unusual characteristic, the researchers took a closer look at the keratin structure of the crown feathers of all three bird species using an electron microscope.
"The golden-crowned manakin ended up with an intermediate keratin structure that does a poor job of making either the brilliant white or the reflective iridescence of the parental species," said Weir.
The golden-crowned manakin likely had duller white or grey feathers early on in its existence as a result of its keratin structure, but eventually evolved yellow feathers as an alternative way to attract females. The end result is a uniquely coloured species.
The golden-crowned manakin lives in an area of the south-central Amazon Rainforest that is about 200 square kilometres and is largely separated from areas where snow- capped and opal-crowned live by wide rivers that the birds are reluctant to cross.
It likely owes its survival as a species on being geographically isolated from its parental species at some point during a past ice age when rainforest coverage contracted, and wide rivers formed natural barriers.
"Without geographic isolation, its very likely this would never have happened because you don't see the hybrids evolving as separate species in other areas where both parental species meet," Weir said.