Washington: Researchers have now discovered the unique way in which a type of Gram-negative bacterium delivers the toxins that make people sick. According to the Binghamton University, State University of New York study, understanding this mechanism may help design better ways to block and eventually control those debilitating toxins.
Authors Assistant Professor Xin Yong, graduate student Ao Li and Associate Professor Jeffrey W. Schertzer published their findings in the Journal of Biological Chemistry. The study looked at how bacteria communicate via the transportation of small molecules. Yong and Schertzer explained that communication molecules stimulate the production of outer membrane vesicles. These small packages then bud off from the surface of the bacterium and contain highly concentrated toxins.
Yong and Schertzer decided to work together on a model to understand more about how the communication molecule inserts itself into the membrane of bacteria in order to physically stimulate the production of these toxin delivery vehicles.
"It's hard to see the molecular detail at that level," explained Schertzer. "But with Dr. Yong's expertise, we were able to build a computational model that helped us understand what actually goes on between individual molecules."
Yong's model allowed them to look at the details of the molecule and understand more about how it interacted with the membrane on a very short timescale. "Our most important finding is that the communication molecule needs to enter the membrane in a very specific way," said Schertzer. "It folds itself like a book, and then will expand once it has entered the membrane."
Schertzer and Yong explained that the communication molecule has both a head and a tail that are known to be flexible, but they did not expect this type of change. In the future, they hope to test what would change in the interaction when the tail is removed or the head is modified.
Learning more about how Gram-negative bacteria communicate with each other can help researchers build a stronger understanding of multi-species interactions and how to eventually control these types of high-risk infections.
