A study conducted at National Institute of Scientific Research suggested that visceral leishmaniasis, a disease caused by infection with Leishmania parasites helps boost the immune system and thwarts the immune system's defenses so that it can settle comfortably into its host.
Responsible for tens of thousands of deaths around the world every year, visceral leishmaniasis holds the unfortunate distinction of being the second leading cause of death by parasitic infection, after malaria. But the team of researchers discovered a mechanism that Leishmania donovani takes advantage of to sustain the infection. It has shown that damage from chronic inflammation induces the death of white blood cells essential to eliminating the parasite.
The findings not only have the potential to lead to possible treatment, they also bring to light a phenomenon that may be shared by other chronic infections.
At the heart of the immune response, CD4 T cells are essential to controlling an infection. This type of white blood cell participates in the immune response by signaling the presence of a pathogen to be eliminated. CD4 T cells produce a molecule called interferon gamma (IFN-?), which activates the cells that destroy pathogens.
In the case of visceral leishmaniasis, CD4 T cells expressing IFN-? (Th1 cells) appear later than expected during infection and in smaller numbers, a phenomenon that had puzzled researchers.
Researchers noticed the presence of a transcription factor is unusual for these cells. A transcription factor is a protein that modulates the activity of one or more genes. The detected factor, IRF-5, is known for its action in innate immunity cells but is role was totally unknown in CD4 T cells.
The new findings show that IRF-5 leads Th1 cells to self-destruction. Tracing the thread of molecular events, the researchers pinned down the trigger for this unexpected cell death: tissue destruction. Following a series of reactions, the destroyed tissue activates previously unknown signals in Th1 cells, causing them to die.
The white blood cells that orchestrate the attack are thus eliminated--not by the parasite but by a biological process of the host--sustaining the chronic infection and protecting the parasite. In fact, this mechanism may also be at play in other chronic infections that lead to inflammation.
It points to a mechanism involving the TLR7 receptor (Toll-like Receptor 7), usually activated in innate immune system cells by pathogen recognition. In the case of chronic inflammation, the cellular residues present after tissue destruction activate TLR7 in Th1 cells. Activation of TLR7 induces expression and activation of IRF-5, which in turn increases expression of DR5 (Death Receptor 5) and caspase 8, two signaling elements leading to cell death. Thus, chronic inflammation induces the death of protective CD4 T cells via the TLR7-IRF-5 cellular pathway.
The findings appeared in the Journal of Cell Reports....